Conformational Studies on C-Phycocyanin from Spirulina platensis
نویسندگان
چکیده
Bile Pigments, Protein Interaction, Conformation, Denaturation The chromophore-protein interactions of C-phycocyanin (C-PC) from Spirulina platensis have been studied by following the partial and complete denaturation with UV-Vis spectroscopy. From comparison with published MO calculations, an elongated conformation of the chromophore is sug gested for native C-PC, a cyclic one for denatured C-PC. By means of partial denaturation, a step wise unfolding of the protein has been demonstrated. The presence of at least two sets of spectro scopically different diromophores is suggested from the partial denaturation and low temperature experiments.
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